| Info. | Vol.17 - No.4 (2023.12.20) |
|---|---|
| Title | In Vitro Assays of Neurite Outgrowth and Synapse Formation Using Thermoplasmonic Ablation Technique |
| Authors | Nari Hong 1,2 , Yoonkey Nam 1,3*
Nari Hong: First author. *Yoonkey Nam ynam@kaist.ac.kr |
| Institutions | 1 Department of Bio and Brain Engineering, Korea Advanced
Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
2 Department of Electrical Engineering and Computer Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea 3 KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea |
| Abstract | A variety of in vitro assays have been designed based on optical imaging to study essential processes for nervous system development, such as neurogenesis and synaptogenesis. However, it is still difficult to assess the developmental processes under culture conditions where uncontrolled neurite growths and numerous neuronal connections occur all at the same time. Moreover, present techniques for in vitro assays lack the ability to induce and evaluate these processes at a specific time point. In this study, we applied a nanoparticle-assisted thermoplasmonic ablation of a cell-repulsive hydrogel to realize assays for two developmental processes: neurite outgrowth and synapse formation. We successfully demonstrated that this technique could guide neurites or make connections at different cultivation times, and the length of growing neurites and the number of forming synapses could be quantified. Our developed method for in vitro assays is expected to be helpful in studying various neurodevelopment processes occurring at different developmental stages. |
| Keyword | Gold nanorod · Agarose hydrogel · Cultured neuron · Thermoplasmonic neural chip platform |
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