| Info. | Vol.17 - No.4 (2023.12.20) |
|---|---|
| Title | Continuous Isolation of Stem-Cell-Derived Extracellular Vesicles (SC-EVs) by Recycled Magnetic Beads in Microfluidic Channels |
| Authors | Haeun Yu1 , Jaejeung Kim 1 , Jianning Yu1,3 , Kyung-A Hyun 1 , Jae-Yol Lim2 , Yeo-Jun Yoon 2 , Sunyoung Park1,4 , Hyo-Il Jung1,4*
Haeun Yu: First author. *Hyo-Il Jung uridle7@yonsei.ac.kr |
| Institutions | 1School of Mechanical Engineering,Yonsei University,50, Yonsei-ro, Seoul 03722, Republic of Korea
2Department of Otorhinolaryngology, Yonsei University College of Medicine, 50-1 Yonsei-ro,Seoul 03722, Republic of Korea 3Department of Biomedical Laboratory Science, College of Software and Digital Healthcare Convergence, Yonsei University, 1 Yonseidae-gil, Wonju 26493, Republic of Korea 4The DABOM Inc., Seoul, Republic of Korea |
| Abstract | Stem cells produce nanosized particles known as extracellular vesicles (SC-EVs), which therapeutically affect stem cells. EVs are more abundantly produced, exhibit better stability, and possess lower immune rejection rates than stem cells. However, the traditional methods of isolating EVs, such as ultracentrifugation, possess limitations that require a complex process and consume more time. Moreover, it is difficult to isolate specific EVs that have target surface proteins that affect regenerative effects. To address these limitations, a new dual-mode horseshoe-shaped orifi ce micromixer (DM-HOMM) chip that can bind antibody-conjugated micromagnetic beads and SC-EVs and sequentially elute specific SC-EVs on the beads using an eluent was developed. For effective elution from the microbead-SC-EV complex, four types of eluents were used to control pH and ionic strength between antibodies and surface proteins in EVs. In addition, we investigated the reusability of antibody-conjugated micromagnetic beads. The beads indicated identical binding efficiencies between the antibodies and specific SC-EVs for three repeated cycles using the dual-mode chip. CD63 + EVs collected by the chip exhibited higher cell viability and regeneration effects than untreated and total EVs. This SC-EVs’ isolation method possesses the potential for targeted therapeutic applications and enhanced regenerative effects. |
| Keyword | Stem cell · Extracellular vesicle · Microfluidic chip · Regeneration · Continuous isolation |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
