| Info. | Vol.17 - No.4 (2023.12.20) |
|---|---|
| Title | A Controlled Transcription-Driven Light-Up Aptamer Amplification for Nucleoside Triphosphate Detection |
| Authors | Deok-Gyu Lee 1,3, Hye-Jin Lim 5, Ha-Yeong Lee 1,2, Dong-Myung Kim 3, Kyung-Ho Lee4, Ju-Young Byun1,2*, Yong-Beom Shin2,6,7*
Deok-Gyu Lee: First author. * Ju-Young Byun bjy8349@kribb.re.kr * Yong-Beom Shin ybshin@kribb.re.kr |
| Institutions | 1 Critical Diseases Diagnostics Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea
2 Department of Nanobiotechnology, KRIBB School of Biotechnology , University of Science and Technology, Daejeon 34113, Republic of Korea |
| Abstract | In this study, we introduced a simple method for detecting nucleoside triphosphates (NTPs) using controlled transcription-driven light-up aptamer amplification. Based on the concentration of target NTP, the light-up aptamer was amplified using a DNA template encoding the Broccoli aptamer sequence through an incomplete transcription mixture (without each NTP). The Broccoli aptamer associated with DFHBI-1T produced significantly enhanced fluorescence signals that could sensitively detect NTPs in a label-free manner. In addition, the proposed assay was useful for quantitatively detecting NTPs in serum. Thus, we expect that this method has great potential for NTP analysis in bioassays and biological researches. |
| Keyword | NTP · Fluorescence signals · DNA template · Broccoli aptamer · DFHBI-1T · Transcription |
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