| Info. | Vol.18 - No.1 (2024.03.20) |
|---|---|
| Title | Colorimetric Detection of Staphylococcus aureus Based on Direct Loop-Mediated Isothermal Amplifi cation in Combination with Lateral Flow Assay |
| Authors | Seungjin Lee 1, Seung Hyeon Reo 1, Seokjoon Kim 1, Seokhwan Kim 1, Eun Sung Lee 1,Byung Seok Cha 1, Jiye Shin 1, Jinjoo Han1,So Min Ahn 2, Han-Seung Shin 2, Ki Soo Park 1*
Seungjin Lee and Seung Hyeon Reo contributed equally to this work. * Ki Soo Park akdong486@konkuk.ac.kr |
| Institutions | 1Department of Biological Engineering, College
of Engineering , Konkuk University , Seoul 05029 ,
Republic of Korea
2Department of Food Science and Biotechnology , Dongguk University-Seoul , 32, Dongguk-Ro, Ilsandong-Gu , Goyang-Si , Gyeonggi-Do 10326 , Republic of Korea |
| Abstract | In this study, Staphylococcus aureus (S. aureus ) was detected using a system that combined direct loop-mediated isothermal amplification (LAMP) and lateral flow assays (LFA). This technology relies on sequence-specific hybridization in LFA; furthermore, it has high specificity and addresses the limitations associated with nonspecific amplification in general colori-metric LAMP. In addition, a direct boiling method was adopted to streamline DNA extraction and enable simple detection. The established technology was used to successfully detect S. aureus at a concentration as low as 10 2 colony-forming unit/ mL, without cross-reactivity with other strains. The practical applicability of this technology was demonstrated by analyzing real samples such as beef jerky, cabbage, and eggshell, which were artificially spiked with S. aureus . This developed system may be beneficial with regard to operational simplicity, short analysis time, and high detection performance, which would enable its application in point-of-care settings and as a novel platform for detecting various pathogens. |
| Keyword | Biosensor · Colorimetric detection · Lateral flow assay · Loop-mediated isothermal amplification · Staphylococcus aureus |
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