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Info. Vol.2 - No.4 (2008.12.20)
Title Comparative Analysis of Gene Expression Patterns after Exposure to Nonylphenol in Human Cell Lines
Authors Moon-Ju Oh1,3,??/sup>, Saswati Paul2,??/sup>, Seung-Jun Kim1,2, Jun-Sub Kim1, Jong-Pil Youn1, Hye-Won Park1,3, Youn-Jung Kim4, Jae-Chun Ryu4, Chul-Woo Lee5, Hyun-Mi Kim5,Kyung-Hee Choi5, Hak-Joo Kim6 & Seung Yong Hwang1,2
Institutions 1GenoCheck Co., Ltd., Hanyang University, Sa 3-dong, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea
2Department of Biochemistry, Hanyang University, Sa 3-dong, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea
3Department of Bio-Nanotechnology, Hanyang University, Sa 3-dong, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea
4Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, Korea
5Environmental Exposure Assessment Division, National Institute of Environmental research, Seo-gu, Incheon, Korea
6Department of Chemicals Assessment, National Institute of Environmental Research, Seo-gu, Incheon, Korea
??/sup>These authors contributed equally to this work Correspondence and requests for materials should be addressed to S.Y. Hwang (syhwang@hanyang.ac.kr)
Abstract Nonylphenol (NP), a byproduct of industrial synthesis, is quite similar to estrogen in structure, and is known as an environmental estrogen that induces estrogenic disturbances. It has been utilized in several industries for the manufacture of skin cleaning materials, kitchen detergents, cosmetics, fabric detergents, and ink binder. Due to its characteristic strong estrogenic potency, NP is capable of disrupting the reproductive hormone system. Exposure to NP for a prolonged period increases the chances of developing breast and lung cancers. In this study, we conducted a comparative study of expression profiles between HK (Human Kidney cell), MCF-7 (Human breast cancer cell), and LNCaP (Human prostate cancer cell) cells treated with NP at the value of IC20, using Agilent whole genome microarrays. After comparative analysis, we detected some specific expression patterns in each of the cell lines. However, the expression patterns from the HK-2 and MCF-7 cells are quite similar. Interestingly, estrogen receptor 1 and 2 genes were downregulated only in MCF-7 cells, whereas the androgen receptor (AR) gene evidenced overexpression in all 3 of the cell lines. The PDZK1 gene, which has been identified as an estrogen-responsive gene, has also been shown to be overexpressed in all 3 of the tested cell lines. The majority of differentially expressed genes in NP treatment were shown to be involved in cell proliferation, transcription, and signaling. These results may establish a framework for nderstanding the mechanisms underlying the toxicity of NP.
Keyword Toxicogenomics, EDCs (Endocrine Disrupting Chemicals), Whole genome microarray, HK (Human Kidney Cell) cell, LNCaP (Prostate cancer cell), MCF (Human Breast Cancer Cell)
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